Capture and analysis of double‐stranded DNA with the α‐hemolysin nanopore: Fundamentals and applications

The α-hemolysin nanopore has attracted much attention as a tool for the single-molecule analysis of DNA due to its potential an ultra-sensitive, specific, and label-free sensing technique. vast majority research with focused on interrogating single-stranded DNA. Nevertheless, structure pore, specifically circa 32.6 cubic nanometer vestibule, is sufficient size short section double-stranded (dsDNA) reside before unzipping into constituents. In this review, we describe past current literature relating rich information that can be obtained from interrogation dsDNA while residing within subsequent voltage-driven Applications have been implemented include sequencing, disease diagnosis, identification epigenetic modifications.